non malignant human mesothelial cell line met5a Search Results


human metastatic melanoma cells  (ATCC)
96
ATCC human metastatic melanoma cells
Human Metastatic Melanoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human mesothelial cells met-5a  (BioResource International Inc)
90
BioResource International Inc human mesothelial cells met-5a
Human Mesothelial Cells Met 5a, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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met5a cells  (ATCC)
96
ATCC met5a cells
Figure 2. Discrimination of high MSLN-expressing cells from low MSLN-expressing cells: a bivalent αMSLN TCE versus its monovalent counterpart. (a) Distribution of MSLN density in three human cell lines used in subsequent experiments: H226 lung cancer cells, and two immortalized nonmalignant mesothelial cell lines, <t>MeT5a,</t> and LP-9/Coriell 86 cells. (b) Mean (SD) MSLN density in the three cell lines shown in a. (c) Dose-dependent cytotoxicity, as determined by LDH assay, after 40 h of exposure to the bivalent αMSLN TCE NM28-2746 (left) and its monovalent counterpart NM28-1872 (right) in co-cultures of the indicated cells and human PBMCs (E:T, 30:1). Values are mean ± SD of replicates from a representative experiment. In co-cultures of CHO cells (which lack MSLN expression) and human PBMCs, NM28-2746 exhibited no cytotoxicity (not shown). The discrimination window (see text) for cytotoxicity in high MSLN-expressing H226 cells versus low MSLN-expressing C86 cells (fold change [ratio] of EC50) was 9.91 (geometric mean of three independent experiments), and the corresponding value for the monovalent NM28-1872 was 1.24 (geometric mean 5 independent experiments). (d) Dose-dependent CD8+ T-cell activation as determined by CD69 flow cytometry in the same experiment as c. Values are mean ± SD of replicates from a representative experiment. (e) Mean (SD) EC50 and Emax values from at least three experiments represented in c and d. (f) Dose-dependent cytokine release in the same experiment as parts c and d.
Met5a Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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simian vacuolating virus 40 sv40 large t antigen construct  (ATCC)
97
ATCC simian vacuolating virus 40 sv40 large t antigen construct
Figure 2. Discrimination of high MSLN-expressing cells from low MSLN-expressing cells: a bivalent αMSLN TCE versus its monovalent counterpart. (a) Distribution of MSLN density in three human cell lines used in subsequent experiments: H226 lung cancer cells, and two immortalized nonmalignant mesothelial cell lines, <t>MeT5a,</t> and LP-9/Coriell 86 cells. (b) Mean (SD) MSLN density in the three cell lines shown in a. (c) Dose-dependent cytotoxicity, as determined by LDH assay, after 40 h of exposure to the bivalent αMSLN TCE NM28-2746 (left) and its monovalent counterpart NM28-1872 (right) in co-cultures of the indicated cells and human PBMCs (E:T, 30:1). Values are mean ± SD of replicates from a representative experiment. In co-cultures of CHO cells (which lack MSLN expression) and human PBMCs, NM28-2746 exhibited no cytotoxicity (not shown). The discrimination window (see text) for cytotoxicity in high MSLN-expressing H226 cells versus low MSLN-expressing C86 cells (fold change [ratio] of EC50) was 9.91 (geometric mean of three independent experiments), and the corresponding value for the monovalent NM28-1872 was 1.24 (geometric mean 5 independent experiments). (d) Dose-dependent CD8+ T-cell activation as determined by CD69 flow cytometry in the same experiment as c. Values are mean ± SD of replicates from a representative experiment. (e) Mean (SD) EC50 and Emax values from at least three experiments represented in c and d. (f) Dose-dependent cytokine release in the same experiment as parts c and d.
Simian Vacuolating Virus 40 Sv40 Large T Antigen Construct, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human pleural mesothelial met5a cell line  (LGC Promochem)
90
LGC Promochem human pleural mesothelial met5a cell line
Figure 2. Discrimination of high MSLN-expressing cells from low MSLN-expressing cells: a bivalent αMSLN TCE versus its monovalent counterpart. (a) Distribution of MSLN density in three human cell lines used in subsequent experiments: H226 lung cancer cells, and two immortalized nonmalignant mesothelial cell lines, <t>MeT5a,</t> and LP-9/Coriell 86 cells. (b) Mean (SD) MSLN density in the three cell lines shown in a. (c) Dose-dependent cytotoxicity, as determined by LDH assay, after 40 h of exposure to the bivalent αMSLN TCE NM28-2746 (left) and its monovalent counterpart NM28-1872 (right) in co-cultures of the indicated cells and human PBMCs (E:T, 30:1). Values are mean ± SD of replicates from a representative experiment. In co-cultures of CHO cells (which lack MSLN expression) and human PBMCs, NM28-2746 exhibited no cytotoxicity (not shown). The discrimination window (see text) for cytotoxicity in high MSLN-expressing H226 cells versus low MSLN-expressing C86 cells (fold change [ratio] of EC50) was 9.91 (geometric mean of three independent experiments), and the corresponding value for the monovalent NM28-1872 was 1.24 (geometric mean 5 independent experiments). (d) Dose-dependent CD8+ T-cell activation as determined by CD69 flow cytometry in the same experiment as c. Values are mean ± SD of replicates from a representative experiment. (e) Mean (SD) EC50 and Emax values from at least three experiments represented in c and d. (f) Dose-dependent cytokine release in the same experiment as parts c and d.
Human Pleural Mesothelial Met5a Cell Line, supplied by LGC Promochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human mesothelium cell line met 5 a  (ATCC)
94
ATCC human mesothelium cell line met 5 a
Figure 2. Discrimination of high MSLN-expressing cells from low MSLN-expressing cells: a bivalent αMSLN TCE versus its monovalent counterpart. (a) Distribution of MSLN density in three human cell lines used in subsequent experiments: H226 lung cancer cells, and two immortalized nonmalignant mesothelial cell lines, <t>MeT5a,</t> and LP-9/Coriell 86 cells. (b) Mean (SD) MSLN density in the three cell lines shown in a. (c) Dose-dependent cytotoxicity, as determined by LDH assay, after 40 h of exposure to the bivalent αMSLN TCE NM28-2746 (left) and its monovalent counterpart NM28-1872 (right) in co-cultures of the indicated cells and human PBMCs (E:T, 30:1). Values are mean ± SD of replicates from a representative experiment. In co-cultures of CHO cells (which lack MSLN expression) and human PBMCs, NM28-2746 exhibited no cytotoxicity (not shown). The discrimination window (see text) for cytotoxicity in high MSLN-expressing H226 cells versus low MSLN-expressing C86 cells (fold change [ratio] of EC50) was 9.91 (geometric mean of three independent experiments), and the corresponding value for the monovalent NM28-1872 was 1.24 (geometric mean 5 independent experiments). (d) Dose-dependent CD8+ T-cell activation as determined by CD69 flow cytometry in the same experiment as c. Values are mean ± SD of replicates from a representative experiment. (e) Mean (SD) EC50 and Emax values from at least three experiments represented in c and d. (f) Dose-dependent cytokine release in the same experiment as parts c and d.
Human Mesothelium Cell Line Met 5 A, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vitro  (ATCC)
99
ATCC vitro
Figure 2. Discrimination of high MSLN-expressing cells from low MSLN-expressing cells: a bivalent αMSLN TCE versus its monovalent counterpart. (a) Distribution of MSLN density in three human cell lines used in subsequent experiments: H226 lung cancer cells, and two immortalized nonmalignant mesothelial cell lines, <t>MeT5a,</t> and LP-9/Coriell 86 cells. (b) Mean (SD) MSLN density in the three cell lines shown in a. (c) Dose-dependent cytotoxicity, as determined by LDH assay, after 40 h of exposure to the bivalent αMSLN TCE NM28-2746 (left) and its monovalent counterpart NM28-1872 (right) in co-cultures of the indicated cells and human PBMCs (E:T, 30:1). Values are mean ± SD of replicates from a representative experiment. In co-cultures of CHO cells (which lack MSLN expression) and human PBMCs, NM28-2746 exhibited no cytotoxicity (not shown). The discrimination window (see text) for cytotoxicity in high MSLN-expressing H226 cells versus low MSLN-expressing C86 cells (fold change [ratio] of EC50) was 9.91 (geometric mean of three independent experiments), and the corresponding value for the monovalent NM28-1872 was 1.24 (geometric mean 5 independent experiments). (d) Dose-dependent CD8+ T-cell activation as determined by CD69 flow cytometry in the same experiment as c. Values are mean ± SD of replicates from a representative experiment. (e) Mean (SD) EC50 and Emax values from at least three experiments represented in c and d. (f) Dose-dependent cytokine release in the same experiment as parts c and d.
Vitro, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vitro - by Bioz Stars, 2026-11
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mesothelial cell line met 5a  (ATCC)
95
ATCC mesothelial cell line met 5a
Figure 2. Discrimination of high MSLN-expressing cells from low MSLN-expressing cells: a bivalent αMSLN TCE versus its monovalent counterpart. (a) Distribution of MSLN density in three human cell lines used in subsequent experiments: H226 lung cancer cells, and two immortalized nonmalignant mesothelial cell lines, <t>MeT5a,</t> and LP-9/Coriell 86 cells. (b) Mean (SD) MSLN density in the three cell lines shown in a. (c) Dose-dependent cytotoxicity, as determined by LDH assay, after 40 h of exposure to the bivalent αMSLN TCE NM28-2746 (left) and its monovalent counterpart NM28-1872 (right) in co-cultures of the indicated cells and human PBMCs (E:T, 30:1). Values are mean ± SD of replicates from a representative experiment. In co-cultures of CHO cells (which lack MSLN expression) and human PBMCs, NM28-2746 exhibited no cytotoxicity (not shown). The discrimination window (see text) for cytotoxicity in high MSLN-expressing H226 cells versus low MSLN-expressing C86 cells (fold change [ratio] of EC50) was 9.91 (geometric mean of three independent experiments), and the corresponding value for the monovalent NM28-1872 was 1.24 (geometric mean 5 independent experiments). (d) Dose-dependent CD8+ T-cell activation as determined by CD69 flow cytometry in the same experiment as c. Values are mean ± SD of replicates from a representative experiment. (e) Mean (SD) EC50 and Emax values from at least three experiments represented in c and d. (f) Dose-dependent cytokine release in the same experiment as parts c and d.
Mesothelial Cell Line Met 5a, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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non malignant mesothelial cell line met 5a  (LGC Standards)
93
LGC Standards non malignant mesothelial cell line met 5a
Figure 2. Discrimination of high MSLN-expressing cells from low MSLN-expressing cells: a bivalent αMSLN TCE versus its monovalent counterpart. (a) Distribution of MSLN density in three human cell lines used in subsequent experiments: H226 lung cancer cells, and two immortalized nonmalignant mesothelial cell lines, <t>MeT5a,</t> and LP-9/Coriell 86 cells. (b) Mean (SD) MSLN density in the three cell lines shown in a. (c) Dose-dependent cytotoxicity, as determined by LDH assay, after 40 h of exposure to the bivalent αMSLN TCE NM28-2746 (left) and its monovalent counterpart NM28-1872 (right) in co-cultures of the indicated cells and human PBMCs (E:T, 30:1). Values are mean ± SD of replicates from a representative experiment. In co-cultures of CHO cells (which lack MSLN expression) and human PBMCs, NM28-2746 exhibited no cytotoxicity (not shown). The discrimination window (see text) for cytotoxicity in high MSLN-expressing H226 cells versus low MSLN-expressing C86 cells (fold change [ratio] of EC50) was 9.91 (geometric mean of three independent experiments), and the corresponding value for the monovalent NM28-1872 was 1.24 (geometric mean 5 independent experiments). (d) Dose-dependent CD8+ T-cell activation as determined by CD69 flow cytometry in the same experiment as c. Values are mean ± SD of replicates from a representative experiment. (e) Mean (SD) EC50 and Emax values from at least three experiments represented in c and d. (f) Dose-dependent cytokine release in the same experiment as parts c and d.
Non Malignant Mesothelial Cell Line Met 5a, supplied by LGC Standards, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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met 5a human mesothelium cells  (Merck & Co)
86
Merck & Co met 5a human mesothelium cells
Figure 2. Discrimination of high MSLN-expressing cells from low MSLN-expressing cells: a bivalent αMSLN TCE versus its monovalent counterpart. (a) Distribution of MSLN density in three human cell lines used in subsequent experiments: H226 lung cancer cells, and two immortalized nonmalignant mesothelial cell lines, <t>MeT5a,</t> and LP-9/Coriell 86 cells. (b) Mean (SD) MSLN density in the three cell lines shown in a. (c) Dose-dependent cytotoxicity, as determined by LDH assay, after 40 h of exposure to the bivalent αMSLN TCE NM28-2746 (left) and its monovalent counterpart NM28-1872 (right) in co-cultures of the indicated cells and human PBMCs (E:T, 30:1). Values are mean ± SD of replicates from a representative experiment. In co-cultures of CHO cells (which lack MSLN expression) and human PBMCs, NM28-2746 exhibited no cytotoxicity (not shown). The discrimination window (see text) for cytotoxicity in high MSLN-expressing H226 cells versus low MSLN-expressing C86 cells (fold change [ratio] of EC50) was 9.91 (geometric mean of three independent experiments), and the corresponding value for the monovalent NM28-1872 was 1.24 (geometric mean 5 independent experiments). (d) Dose-dependent CD8+ T-cell activation as determined by CD69 flow cytometry in the same experiment as c. Values are mean ± SD of replicates from a representative experiment. (e) Mean (SD) EC50 and Emax values from at least three experiments represented in c and d. (f) Dose-dependent cytokine release in the same experiment as parts c and d.
Met 5a Human Mesothelium Cells, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell line met-5a  (EuroClone)
90
EuroClone cell line met-5a
Effect of GEOCYDO on cell cycle progression. (A) Distribution of MSTO cell population during the cell cycle after 16, 24 and 48 h of treatment with 500 µg/ml GEOCYDO (+) analyzed by flow cytometry. Cells treated with vehicle only (0.1% DMSO) were used as a control (−). (B) Histograms of the cell cycle distribution in MSTO, NCI and Mes2 cells. Data are presented as the mean ± standard deviation (n=3). MSTO, MSTO-211H; NCI, NCI-H2452; Mes2, Ist-Mes2; GEOCYDO, Geodia cydonium extract; P3, G 0 /G 1 cell population; P4, S cell population; P5, G 2 /M cell population.
Cell Line Met 5a, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pleural mesothelial cells  (ATCC)
99
ATCC pleural mesothelial cells
Effect of GEOCYDO on cell cycle progression. (A) Distribution of MSTO cell population during the cell cycle after 16, 24 and 48 h of treatment with 500 µg/ml GEOCYDO (+) analyzed by flow cytometry. Cells treated with vehicle only (0.1% DMSO) were used as a control (−). (B) Histograms of the cell cycle distribution in MSTO, NCI and Mes2 cells. Data are presented as the mean ± standard deviation (n=3). MSTO, MSTO-211H; NCI, NCI-H2452; Mes2, Ist-Mes2; GEOCYDO, Geodia cydonium extract; P3, G 0 /G 1 cell population; P4, S cell population; P5, G 2 /M cell population.
Pleural Mesothelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 2. Discrimination of high MSLN-expressing cells from low MSLN-expressing cells: a bivalent αMSLN TCE versus its monovalent counterpart. (a) Distribution of MSLN density in three human cell lines used in subsequent experiments: H226 lung cancer cells, and two immortalized nonmalignant mesothelial cell lines, MeT5a, and LP-9/Coriell 86 cells. (b) Mean (SD) MSLN density in the three cell lines shown in a. (c) Dose-dependent cytotoxicity, as determined by LDH assay, after 40 h of exposure to the bivalent αMSLN TCE NM28-2746 (left) and its monovalent counterpart NM28-1872 (right) in co-cultures of the indicated cells and human PBMCs (E:T, 30:1). Values are mean ± SD of replicates from a representative experiment. In co-cultures of CHO cells (which lack MSLN expression) and human PBMCs, NM28-2746 exhibited no cytotoxicity (not shown). The discrimination window (see text) for cytotoxicity in high MSLN-expressing H226 cells versus low MSLN-expressing C86 cells (fold change [ratio] of EC50) was 9.91 (geometric mean of three independent experiments), and the corresponding value for the monovalent NM28-1872 was 1.24 (geometric mean 5 independent experiments). (d) Dose-dependent CD8+ T-cell activation as determined by CD69 flow cytometry in the same experiment as c. Values are mean ± SD of replicates from a representative experiment. (e) Mean (SD) EC50 and Emax values from at least three experiments represented in c and d. (f) Dose-dependent cytokine release in the same experiment as parts c and d.

Journal: OncoImmunology

Article Title: An engineered T-cell engager with selectivity for high mesothelin-expressing cells and activity in the presence of soluble mesothelin

doi: 10.1080/2162402x.2023.2233401

Figure Lengend Snippet: Figure 2. Discrimination of high MSLN-expressing cells from low MSLN-expressing cells: a bivalent αMSLN TCE versus its monovalent counterpart. (a) Distribution of MSLN density in three human cell lines used in subsequent experiments: H226 lung cancer cells, and two immortalized nonmalignant mesothelial cell lines, MeT5a, and LP-9/Coriell 86 cells. (b) Mean (SD) MSLN density in the three cell lines shown in a. (c) Dose-dependent cytotoxicity, as determined by LDH assay, after 40 h of exposure to the bivalent αMSLN TCE NM28-2746 (left) and its monovalent counterpart NM28-1872 (right) in co-cultures of the indicated cells and human PBMCs (E:T, 30:1). Values are mean ± SD of replicates from a representative experiment. In co-cultures of CHO cells (which lack MSLN expression) and human PBMCs, NM28-2746 exhibited no cytotoxicity (not shown). The discrimination window (see text) for cytotoxicity in high MSLN-expressing H226 cells versus low MSLN-expressing C86 cells (fold change [ratio] of EC50) was 9.91 (geometric mean of three independent experiments), and the corresponding value for the monovalent NM28-1872 was 1.24 (geometric mean 5 independent experiments). (d) Dose-dependent CD8+ T-cell activation as determined by CD69 flow cytometry in the same experiment as c. Values are mean ± SD of replicates from a representative experiment. (e) Mean (SD) EC50 and Emax values from at least three experiments represented in c and d. (f) Dose-dependent cytokine release in the same experiment as parts c and d.

Article Snippet: H226 and MeT5A cells were obtained from ATCC (#CRL-5826; 9444), and C86 mesothelial cells from Coriell Institute (#AG07086).

Techniques: Expressing, Lactate Dehydrogenase Assay, Activation Assay, Flow Cytometry

Effect of GEOCYDO on cell cycle progression. (A) Distribution of MSTO cell population during the cell cycle after 16, 24 and 48 h of treatment with 500 µg/ml GEOCYDO (+) analyzed by flow cytometry. Cells treated with vehicle only (0.1% DMSO) were used as a control (−). (B) Histograms of the cell cycle distribution in MSTO, NCI and Mes2 cells. Data are presented as the mean ± standard deviation (n=3). MSTO, MSTO-211H; NCI, NCI-H2452; Mes2, Ist-Mes2; GEOCYDO, Geodia cydonium extract; P3, G 0 /G 1 cell population; P4, S cell population; P5, G 2 /M cell population.

Journal: Oncology Letters

Article Title: Organic extract of Geodia cydonium induces cell cycle block in human mesothelioma cells

doi: 10.3892/ol.2022.13406

Figure Lengend Snippet: Effect of GEOCYDO on cell cycle progression. (A) Distribution of MSTO cell population during the cell cycle after 16, 24 and 48 h of treatment with 500 µg/ml GEOCYDO (+) analyzed by flow cytometry. Cells treated with vehicle only (0.1% DMSO) were used as a control (−). (B) Histograms of the cell cycle distribution in MSTO, NCI and Mes2 cells. Data are presented as the mean ± standard deviation (n=3). MSTO, MSTO-211H; NCI, NCI-H2452; Mes2, Ist-Mes2; GEOCYDO, Geodia cydonium extract; P3, G 0 /G 1 cell population; P4, S cell population; P5, G 2 /M cell population.

Article Snippet: Human mesothelioma cell lines MSTO and NCI, and the human mesothelial cell line MeT-5A were grown in RPMI supplemented with 10% FBS (both from Euroclone SpA), glutamine (2 mM), sodium pyruvate and antibiotics (0.02 IU/ml penicillin and 0.02 mg/ml streptomycin).

Techniques: Flow Cytometry, Control, Standard Deviation